Freezing of stallion semen: I – In vitro evaluation of motility and acrosin activity in sperm cells cryopreserved under different glycerol concentrations
نویسندگان
چکیده
منابع مشابه
Proacrosin-acrosin activity in capacitated and acrosome reacted sperm from cryopreserved bovine semen.
Acrosin activity is associated with normal fertility in human and bovine spermatozoa. The aim of the study was to determine the variation of the enzyme activity in the proacrosin-acrosin system in capacitated and acrosome reacted cryopreserved bovine sperm. Enzyme activity was assessed spectrophotometrically using N-alpha-benzoyl-DL-arginine p-nitroanilide (BAPNA) as specific substrate for acro...
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چکیده: یکی از دوره های مهم تاریخی ایران به لحاظ تأمین استقلال ملی مذهبی و حتی تأثیر آن بر فرهنگ و مذهب ایرانیان، دوره صفویه است. رسمیت دادن و رواج مذهب شیعه توسط شاه اسماعیل اول، یکی از مهمترین اقدامات این دولت محسوب می شود. بنابراین برای اجرای این سیاست، وی منصب صدارت را به عنوان منصبی مذهبی- حکو متی ایجاد کرد .این منصب از دوره ی تیموریان ایجاد شده بود ولی در اواخر این دوره اهمیت بیشتری یافت...
15 صفحه اولInvestigation of Different Glycerol and Egg Yolk Concentration on Freezing Bakhtiari Ram Semen
Purpose: Glycerol has been the most widely used cryopreservation agent for spermatozoa and a wide range of factors affects its action on sperm viability and fertilizing capacity. Moreover egg yolk is a common constituent of semen diluents, protects the spermatozoa against cold shock and confers protection during freezing and thawing .The aim of this study was to investigate of different glycero...
متن کاملFreezing stallion semen with the new Cáceres extender improves post thaw sperm quality and diminishes stallion-to-stallion variability.
Ejaculates from 7 stallions were split and simultaneously frozen in three different extenders, INRA 96 egg yolk glycerol, Ghent and the newly developed extender Caceres. After thawing, samples were evaluated for motility (CASA system) sperm membrane integrity and early membrane changes (YoPro-1/Eth staining), acrosome integrity (FICT-PNA), and mitochondrial membrane potential (JC-1) (flow cytom...
متن کاملSperm Characteristics in Cryopreserved Buffalo Bull Semen and Field Fertility
Semen quality and its relationship to fertility are major concern in animal production. The aim of this study was to assess the relationship between frozen thawed sperm characteristics and fertility following in vivo fertility through Artificial Insemination(AI). Semen samples were collected from four buffalo bulls. Semen volume and sperm concentration appeared to be significantly different (P0...
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ژورنال
عنوان ژورنال: Pferdeheilkunde Equine Medicine
سال: 2018
ISSN: 0177-7726
DOI: 10.21836/pem20180109